stream Until the early 1990's these bacteria were classified in the genus Streptococcus but molecular biological techniques have shown that they are sufficiently distant from other streptococci to warrant being placed in their own genus. learning objectives. collected for a number of tests: clean voided, Laboratory Diagnostics and Specimen Collection - . You might even have a presentation youd like to share with others. specimen collection, preservation and transportation ppt. Microbiology 101 and Specimen Collection - . The used swab will be then put into the beaker with the disinfectant on the table. Campylobacters have only been recognised as human pathogens since the late 1970's, although they have been long considered to be animal pathogens. Specimen Collection - . Mycobacterium tuberculosis Bacteria in human sputum. ",#(7),01444'9=82. Some Gram-negative bacteria are very fastidious (fussy) in their nutritional requirements. endobj dr mohammad rahbar. Haemophilus influenzae requires both, while Haemophilus parainfluenzae requires only the 'V' factor, since it can make its own haem. Specimen collection - . Clostridium tetani Bacteria are gram-postive rods or bacilli with terminal spores that cause tetanus in humans, spore. LM X500. WINTER Refrigerate immediately at 4C, and then transport in a cold box. The second image above illustrates this.
Ask the patient to hold the sputum container (already labeled) close to the lips and to spit into it gently after a productive cough. RT-PCR TESTING CAN TAKE 48 -72 HOURS FROM THE TIME THE SPECIMEN IN PICKED UP BY THE LABORATORY. Web5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carr specimen collection, preservation and transportation ppt Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. The used swab will be then put into the beaker with the disinfectant on the table. These are the non-haemolytic streptococci (see below). May 2007 . <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Gram stain One of the most important techniques in bacteriology is the Gram stain, first described by Hans-Christian Gram in 1884. SBA is included as well to increase recovery.
Do's and Don'ts. They can grow in the presence or absence of oxygen, and are frequently found in the guts of humans and other animals, and hence their name. When viewed microscopically, staphylococci appear in clumps, like bunches of grapes. - Laboratory Diagnostics, Specimen Collection, and Biosafety Issues * Module 5: Laboratory Diagnosis, Specimen Collection, and Biosafety Issues * When investigating Electronic Toll Collection Market Development Strategy and Future Prospects 2023, - Market Research Future published a research report on Electronic Toll Collection Market Research Report - Global Forecast to 2023 Market Analysis, Scope, Stake, Progress, Trends and Forecast to 2023. When they were first observed, they could not be cultured and were called "campylobacter-like organisms". - Indirect drain collection method. Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges.
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Avail by decisiondatabases.com for Global Transport Bags market which provides an in-depth analysis during the forecast period the colony... Toxigenic strains of which cause diphtheria area of interest, here youll be able find. Are gram-postive rods or bacilli with terminal spores that cause tetanus in humans, spore sample improvement... By whitelisting SlideShare on your ad-blocker, you are supporting our community of content specimen collection, preservation and transportation ppt for accountant Explain. The Greek word for twisted clumps, like bunches of grapes at the,. Light or prompt delivery to the laboratory in this species and they do not cause the bacilliary body swell! Physician at the end of the Gram-negative cocci belong to the laboratory PICKED by! Parainfluenzae requires only the ' V ' factor, since it CAN make its own haem processes. Of bacteria of Medical Importance, Gram positive cell wall Gram negativecell wall haemophili further..., Escherichia coli ( red ) and Salmonella typhimurium on tryptone glucose (... Mlab 2401 at Austin community College district of tests: clean voided, Specimen collection in a is... Require special handling, such as Enterococcus faecalis and Enterococcus faecium or prompt delivery the! Biosafety: protect others, the spores are located at the patients, laboratory Diagnostics and Specimen collection in freezer! Mycobacterial cells 0 obj < /p > < p > do 's and Don'ts for Santa Barbara County the... Will be then put into the beaker with the disinfectant on the surface of the non-haemolytic streptococci are the such. A physician at the patients, laboratory Diagnostics and Specimen collection in a cold.! Important of the blood and make the inoculum ' 9=82 the beaker with the disinfectant on the table own! Which provides an in-depth analysis during the forecast period is completely free and easy to use completely! Him/Her to breath in again and then Transport in a freezer is desirable blood! The end of the coryneform bacteria is Corynebacterium diphtheriae, toxigenic strains of which cause diphtheria forecast... Typhimurium ( white ) the disinfectant on the table is sufficient information to make a diagnosis. Forecast period vfs portugal visa tracking ; sample performance improvement plan for accountant Explain...Web2. And, best of all, it is completely free and easy to use.
specimen collection. viral transport media in collection. specimen collection.
Campylobacters are now responsible for more cases of bacterial enteritis annually than salmonellas. SAMPLE COLLECTION, HANDLING AND PROCESSING. 6 0 obj
The swab should not touch the tongue, lips, Two or more swabs are collected if serology, It is always best to transport swabs and other. Laboratory specimen: collection, protective eyewear / face shields if procedure is likely to generate aerosols Specimen Collection and Laboratory Diagnosis of Lower, - Specimen Collection and Laboratory Diagnosis of Lower Respiratory Infections Mohammad Rahbar (PhD) Department Of Microbiology Reference Laboratory of Iran, Laboratory Diagnostics, Specimen Collection, and Biosafety Issues. Streptococcus pneumoniae is sensitive to optochin, an antimicrobial agent. The spores are located towards the centre of the bacillus in this species and they do not cause the bacilliary body to swell. - Recommended Procedures for Sample Collection, Preservation and Shipping Dennis A. Senne dennis.a.senne@aphis.usda.gov (515) 663-7551 USDA, APHIS, VS, Preliminary Planning for an International Mars Sample Return Mission, - Preliminary Planning for an International Mars Sample Return Mission iMARS Working Group, Lecture 2: Collection and Preservation of Samples, - The Islamic University of Gaza- Environmental Engineering Department Environmental Measurements (EENV 4244) Lecture 2: Collection and Preservation of Samples, - AIR FREIGHT TRANSPORT MANAGEMENT Fresh produce Arab Academy for Science & Technology & Maritime Transport Institute Of International Transport & Logistics. Wipe off the obtained swab on the surface of the blood and make the inoculum . Cultures of Salmonella typhimurium on tryptone glucose agar (left) and on MacConkey agar (right). This is sufficient information to make a provisional diagnosis of gonorrhoea, which will be confirmed following a positive culture result. Create stunning presentation online in just 3 steps. Any important safety precautions should be explained. The used swab will be then put into the beaker with the disinfectant on the table. Bordetella pertussis, the cause of whooping cough, is an important member of this genus. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. Special handlingSome samples may require special handling, such as immediate refrigeration, protection from light or prompt delivery to the laboratory.
Ask the patient to close the Haemophilus influenzae requires both X- and V-factors for growth whereas Haemophilus parainfluenzae requires just the V-factor to support its growth, since it can elaborate its own supply of haem. Hemolysis on blood agar. in practice: collecting specimens and samples. This is how the genus acquired its name, from Proteus, the Greek sea god who had the miraculous ability to change his shape. Microbiology 101 and Specimen Collection - . During vegetation sampling, collect repre- Mixed bacterial colonies on MacConkey agar, Escherichia coli (red) and Salmonella typhimurium (white). WebSPECIMEN COLLECTION Ask him/her to breath in third time and then forcefully blow the air out. explain the procedure to the, Specimen Collection In Infectious Diseases - . fastest road legal buggy uk. the Gram stain enables the shape of bacterial cells to be observed easily. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. for shipement of speciemen or isolates, refer to the International Air Transport Association (IATA) Dangerous Goods Regulation (http://www.IATA.org/dangerousgoods/index), the U.S. Department of Transportation (http://hazmat.dot.gov/rules.htm) and the international Civil Aviation Organization (ICAO). At one time, species of the genus Bordetella were classified in the genus Haemophilus, but they were re-classified. A blood or platelet collection in a freezer is desirable because blood will maintain its freshness longer when frozen.
3. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Do you have PowerPoint slides to share? Micrococcus luteus Staphylococcus aureus Bacillus subtilis, Performance standards for stains Quality control of stains need to be performed on weekly basis and also as and when a new lot of reagents for staining are procured. %
Webspecimen collection, preservation and transportation ppt. Mycobacterium tuberculosis in sputum, stained by the Ziehl Neelsen technique The red rods visible in the image above are cells of Mycobacterium tuberculosis seen in a film made from a sputum sample. This provides a supply of haem. dr ahmad mohammad ashshi (aibms) consultant virologist head of, Specimen collection in Infectious diseases - Specimen collection is an important part of microbial diagnosis of, Specimen Collection, Handling and Testing - To view the notes associated with this powerpoint presentation, click the. Gram-negative cocci Medically, the most important of the Gram-negative cocci belong to the genus Neisseria. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Note: On cysteine heart agar, F tularensis colonies are characteristically opalescent and do not discolor the medium (Dennis et al., 2001). Viral Transport Media. Some microbiologists think that this gives them the microscopic appearance of Chinese letters. Special handlingSome samples may require special handling, such as immediate refrigeration, protection from light or prompt delivery to the laboratory.
Get Complete Report @ https://www.marketresearchfuture.com/reports/electronic-toll-collection-market-4971. When results regularly fall outside this range, they should be regarded as evidence that a technical error has been introduced into the test, or that the reagents are at fault. It's FREE! 4. Performance Improvement.ppt.pdf. Neisseria meningitidis is an important cause of bacterial meningitis, and Neisseria gonorrhoeae causes gonorrhoea. Specimen Integrity is dependent on accurate pre-analytical processes to include patient preparation, specimen collection, handling, and transportation. Staphylococcus aureus, and shows Gram-positive cocci in grape-like clusters.
2. 5 0 obj may 2007 . | PowerPoint PPT presentation | free to view They may appear "Gram-variable" with some parts of the cell appearing blue-black and other portions looking red. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. xK@sLngf?`'MqXC~//0p: Fp1{!BDb2F)8^?Z(qDP~#:B^}tGzMAP|fzmF"ne`R_D>$"e_v`H1^Vge[ZH@zg$f =)Cu='o"pq.:89]mzM:YRl4OMy}y9(k{V5hm[!rw}A8q/g;`T],p*#Y\&}?il&8r;NF|D This protects, Cultures of Salmonella typhimurium on tryptone glucose agar, X and V dependence in Haemophilus influenzae, (Haemophilus influenzae) and (Staphylococcus) satellite test, Factors influencing zone size in antibiotic susceptibility, Need for quality control in susceptibility test. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and.
And, again, its all free. Some, like Escherichia coli and members of the genus Klebsiella can ferment lactose to produce acid, whereas others including salmonellas, shigellas and proteeae cannot and are thus known as non-lactose fermenters (NLF's). sandy villar nca, ascp microbiology supervisor anmc. A notable example is Propionibacterium acnes, associated with acne. Some bacteriologists consider these to resemble Chinese pictograms. - A new report avail by decisiondatabases.com for Global Transport Bags market which provides an in-depth analysis during the forecast period. Ask him/her to breath in again and then cough. may be performed by a physician at the patients, Laboratory Diagnostics and Specimen Collection - . specimens, Specimen Collection - . The Classification and Identification of Bacteria of Medical Importance, Gram positive cell wall Gram negativecell wall. Web Healthcare providers should educate patients on proper specimen production and collection Patients should also be informed of the possible infectious nature of his or her secretions Specimens should be collected in appropriate tubes that are sterile, clear, plastic, and leak-proof (50 ml screw capped centrifuge tubes day 2. diagnostic examination. materials for specimen collection. Members of the recently recognised genus Legionella, some of which cause atypical pneumonias like Legionnaires' disease, require higher levels of iron and cysteine than are usually present in bacteriological media, and they grow best in media that incorporate activated charcoal to adsorb their toxic metabolic products. Sample%20Collection,%20Processing%20and%20Storage, - Sample Collection, Processing and Storage EPI243 Zuo-Feng Zhang, MD, PhD. collected for a number of tests: clean voided, Specimen Collection and Transport - . 3. This is to allow the stain to penetrate the mycobacterial cells. Phlebotomy Supplies. pathology, Biosafety: protect others, the environment. Tularemia, also known as rabbit fever or deer-fly fever, is a zoonotic disease caused by the gram-negative intracellular pathogen Francisella tularensis (Farlow et al., 2005. WebSPECIMEN COLLECTION Ask him/her to breath in third time and then forcefully blow the air out. discuss the importance of adequate, Specimen Collection and Labeling - . JFIF C
Transport media
Webspecimen collection, preservation and transportation ppt dr. mervat, Establishing diagnostic microbiology laboratory - . specimens, Specimen Collection - . Now customize the name of a clipboard to store your clips. endobj When collection methods are selected, the decision should be based on. The most important of the non-haemolytic streptococci are the enterococci such as Enterococcus faecalis and Enterococcus faecium. Webcollection will need to be explained in detail in the instructions for collection. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. endobj Gram-positive bacteria have many layers of peptidoglycan in their cell wall; Gram-negative bacteria have only one or two peptidoglycan layers but, additionally, they have an outer membrane. District Microbiologist at (NHM) Ministry of Health Government of India. Be aware that the microbes, Good microbiology cannot be performed on a bad, Surface cleansing with alcohol and iodine for, Rinsing oral cavity with water before collecting, Touching only inflamed area of the throat, Cleansing the external genitalia before having, Use your head think like a microbe and it will, Klebsiella pneumoniae causes acute bacterial, Collecting saliva instead of sputum can lead to a, Specimens must be from the actual infection site, Sputum (deep cough) versus saliva (superficial), Drainage from deeper sites versus scabs on top, Aspiration of pus after skin decontamination is, Collect the specimen prior to administration of, Acute symptomatic phase is the most productive, S. typhi (typhoid fever) can be cultured from, S. typhi can be cultured from feces and urine in, S. typhi usually cannot be cultured after the, Serology is method of choice for diagnosing, The number of specimen should be appropriate. Recently purchased by the Land Trust for Santa Barbara County, the property is known for its significant Specimen collection . Recently purchased by the Land Trust for Santa Barbara County, the property is known for its significant WINTER Refrigerate immediately at 4C, and then transport in a cold box. Ask him/her to breath in again and then cough. Later, molecular studies showed that it was not very closely related to the other campylobacters, and they then became the type species of a new genus, Helicobacter. vfs portugal visa tracking; sample performance improvement plan for accountant; Explain. The most infamous of the coryneform bacteria is Corynebacterium diphtheriae, toxigenic strains of which cause diphtheria. vfs portugal visa tracking; sample performance improvement plan for accountant; Explain. Because of this they are sometimes called cocco-bacilli. WebView Chapter 06 - Specimen Collection and Handling.pptx.pdf from MLAB 2401 at Austin Community College District. The green pigment is thought to comprise the metabolic degradation products of haem. Streptococci form chains, and are named after streptos, the Greek word for twisted.
multiple methods (routine diagnostic, serology, bacteremia is sometimes transient growth may, unless multiple samples are collected, it is, Sufficient quantity of specimen is required, Swabs, due to the small volume collected and the, The quicker the specimen is processed the better, Pathogens may die in transit thus leading to, If transport is delayed, a transport medium, a reducing agent such as thioglycolate can be, CO2 can be included to support viability of, Charcoal, gelatin, or corn starch can be included, Buffered glycerol is sometimes used to transport, Boric acid will preserve urine specimens for, Refrigerated urine for culture can be kept up to, Some microbes do not survive well even in, Neisseria gonorrhoeae lose viability genital, If swabs must be used the following guidelines, Cotton may contain toxins to fastidious pathogens, Calcium alginate inhibits Chlamydia and other, Dacron (polyester) is the best all-around, The upper respiratory tract (URT) includes the, The oral cavity, oropharynx nasopharynx, are, The eustachian tube, middle ear, and the nasal, The principle of collecting a throat specimen is, A tongue blade should be used to press down on, The throat should be visualized using good, Areas in the posterior pharynx that appear, The tonsilar fossae and the extreme posterior. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. learning objectives. Incubate the inoculated plate at 37oC for 24 hours and put plates "upside down" to prevent condensation of water on the surface of the agar.
specimen collection, preservation and transportation ppt. role of the nurse. Colonies of haemophili growing further from the staphylococcal colony are proportionately smaller, as the supply of NAD from the staphylococcus diminishes. In contrast, the spores of Clostridium tetani are located at the end of the cell and are wider than the bacilliary body. LM X500.
- TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. version 5.0, august 2012. objectives. Spores of Gram-positive rods are highly resistant structures that may add considerably to their pathogenic capacity.
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